The uniformity of the incident electromagnetic radiofrequency fields (RF) is an important factor that can influence the results in biological in vivo and/or in vitro exposure experiments using animals and humans or their cells. The International Electrotechnical Commission (IEC) has published IEC 61000-4-20 standard which defined field uniformity criteria for emission and immunity testing in a defined region in transverse electromagnetic (TEM) waveguides. In this paper, we present a numerical analysis method to determine aperture field uniformity in biological experiments according to IEC 61000-4-20:2010 standard. With the numerical analysis method, the uniformity of electromagnetic field can be analyzed in Cartesian coordinates system by aperture-field method (AFM). Then, with the simultaneous application of AFM and the field uniformity criteria defined by IEC 61000-4-20:2010, the two functions can be programmed to evaluate the field uniformity in region of interest (ROI) which can then be meshed into the given observation points where biological examples are exposed to RF. At the specified position of ROI along z far from the aperture of the WR-430 rectangular open-ended waveguide, the field and the minimum uniform distances vs. frequencies can be calculated by AFM. Thus, the results of the numerical analysis method can be applied to design the exposure setups for biological experiments with the field uniformity required in ROI.
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