Light sheet microscope is a versatile imaging tool for high imaging speed and signal to noise ratio (SNR). In this type of system, the illumination is perpendicular to the direction of detection. Due to its structural feature of perpendicular detection, the SNR is comparable to total internal reflection fluorescence (TIRF) microscopy. Therefore, the perpendicular detection system is of great application prospect. In this paper, we develope a compact optical perpendicular detection system, which can not only be utilized to measure fluorescence with high SNR, but also capture a fluorescent image of flow fluorophore.
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